刘剑, 李南林
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肇庆市林业局森林病虫害防治检疫站 广东肇庆 526040
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摘要: |
竹材木质素的降解一直是制约竹材资源利用的重要影响因素。文章通过对49 份腐烂的竹材样品进行平板划线分离纯化得到74 株真菌,利用PDA- 愈创木酚变色及PDA- 苯胺蓝退色反应,初步筛选出具有木质素降解酶能力的4 株木质素降解菌,通过酶活力测定对初筛得到的4 株木质素降解菌进行复筛,发现FG-22 菌株具有较好木质素降解能力,对FG-22 降解菌株进行形态学和分子生物学鉴定,确定FG-22 为杂色云芝(Trametes versicolor)。
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关键词:
木质素降解菌;竹材;筛选;鉴定
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DOI:
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分类号:
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Screening and Identification of Bamboo Lignin Degradation Fungus |
LIU Jian, LI Nanlin
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Zhaoqing Forestry Bureau Forest Pest Control and Quarantine Station,Zhaoqing
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Abstract: |
The degradation of bamboo lignin has always been an important factor influencing the utilization of bamboo resources. Seventy-four fungi were isolated by streaking and purifying 49 samples of rotten bamboo. The lignin-degrading fungi producing lignin-degrading enzyme activity were screened by PDA-guaiacol phenol discoloration and PDA-aniline blue fading reaction. Four strains of lignin-degrading fungi were screened by enzyme activity assay. It was found that FG-22 fungus had better lignin degradation ability, and the FG-22-degrading fungus was identified by morphological identification and molecular biology to determine FG-22 is Trametes versicolor.
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Key words:
lignin-degrading fungus;bamboo;screen;identification
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